In india it supports livelihood of million of people. It ensures they are true to type and free from pests and diseases. Micropropagationomicsgroupjournal of biotechnology and. Asiapacific consortium on agricultural biotechnology apcoab. Banana is an example of a plant that is vegetatively propagated by suckers. Some basic elements of banana cultivation banana musa acuminata or musa balbisiana is a giant herbaceous plant and is complete in its morphological. Application of tissue culture techniques for genetic up gradation of economically important plants has been reported. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods. A meristem produces both a stern and a root and the structure is generally referred to as. Seed propagation is general in wild species that are diploid and go through normal meiosis, seed set. Musa that fall into one of two subgroups pillay et al. Managing moko disease of banana in latin america and the caribbean yellow zone corresponds to plants growing within a radius of 5 m around the red zone 1. Banana production, first published by shep in 2009, revised by shep plus in 2019 ver. Banana growing in the florida home landscape1 edis.
Serial removal of pseudo stem sheaths, using sterilized cutting tools, this should be concurrent with scarification of buds. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation. Banana cultivation in india banana is a globally important fruit crop with 97. Micropropagation of banana using a liquid culture system. However, the rate of propagation in nature is rather slow. Production of banana is constrained by many biotic as well as abiotic factors. William 253,700 apple 28,800 cooking banana sava 3,000 canadian cold tolerant variety 1,000 total 286, 500.
The micropropagation of chestnut via axillary shoots involves four stages. Explants were taken from young suckers of field grown plants of var. Banana suckers selected are excised to obtain approximately 4 inches 10 cm of inner pseudostem tissue containing the banana meristem, as described in detail in fig. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot. Introduction the cultures of banana, banana tree and topocho, pertaining to the family of the musa. Agro climetic conditoin is suitable for tissue culture banana cultivation bunches are uniform, big finger size and average 140150 fingers per bunch. Virus testing protocols for banana adopted at nrcb 51 iv. A meristem produces both a stern and a root and the structure is generally referred to as a corm or bulb. Banana micropropagation medium has been developed for the in vitro propagation of musa species, family musaceae. Tissue culturing is the popular process of creating clones from a source plant. Effective sterilization protocol for micropropagation of musa coccinea musa spp reza farzinebrahimi 1, kamaludin rashid 2, rosna mat taha 1, jamilah syafawati yaacob 1 1 institute of biological. Conventional micropropagation techniques, however, may be too costly for commercial purposes.
Virus free plant sub millimeter portion of shoottip stock plant virus free. Micropropagation of banana using a liquid culture system chifumi nagai hawaii agriculture research center august 24, 2007 38th annual hawaii banana industry association conference. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such. Check with a department of agriculture and food quarantine officer before bringing tissue culture material into western australia. Banana is a crop which has dual propagation abilities, asexual through suckers and sexual through seeds. Some basic elements of banana cultivation banana musa acuminata or musa balbisiana is a giant herbaceous plant and is complete in its morphological characteristics, i. The production of disease free planting material of banana by meristem culture technique is an urgent need. An experiment has been designed to develop a suitable and efficient protocol for plant regeneration in banana through micro propagation. In an effort to develop a banana as a manifestation of peoples food menu diversification, processing bananas into banana flour are the main alternatives that have good. Bananasmusaspp, including plantains and cooking bananas, are among the most important horticultural crops in tropical and subtropical regions of the world.
In an effort to develop a banana as a manifestation of peoples food menu. Bananas are grown in more than 150 countries, producing 105 million tonnes of fruit. The time between planting a banana plant and the harvest of the banana bunch goes from 9 to 12 months. An overview 325 ingredient for different functional and convenience foods like cookies17 and chips18. The formulation is a nutrient blend of inorganic salts, amino acid and vitamins. This paper reports progress in successfully inducing plantlets from shoot tip explants of banana.
The technique has been applied to a substantial number of commercial vegetatively propagated plant species. Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana. Crop the banana is not a tree but a high herb that can attain up to 15 feet of height. Of the various culture media that have been assayed for micropropagation of. Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. This is typically the case for system files in old operating systems, file types from long discontinued software, or previous versions of certain file types like documents, projects. Collect at least two suckers from each plant source, one for micropropagation and the other for a nursery farm for future keiki needs. K including mg and ca nutrient requirement at different growing stages 2. Banana is a staple food crop for millions of people, vital to food security and ranks in the worlds top four food products roux et al. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its. Joy and others published protocol for micropropagation of banana find, read and cite all the research you need on researchgate. Tissue culture is the best method for propagating bananas. Plant propagation by tissue culture is known as micropropagation. Culture media used at different stages of banana micropropagation 50 iii.
There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. The cultivation guidelines are available in english, spanish and german for the following crops. Aparajita ghosh, junior statistical officer, directorate of marketing and inspection, branch head office, nagpur. Bananas do not grow from a seed but from a bulb or rhizome. Propagation of banana introduction banana is a tropical crop, requiring warm, humid climate. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. In vitro micropropagation of banana 647 as regards yield performance, tissue cultured plants have been reported to produce 39% higher yield than plants from sword suckers pradeep et al. Micropropagation stages, types, applications, advantages, limitations. They are cultivated in areas of about 9 millions hectares spread throughout more than 100 countries, with an annual production of around 89 million metric tons fao, 2000. Banana occupy 20% area among the total area under crop in india. The ideal soil should be well drained but have good water retention capacity. Processing of banana flour using a local banana as raw.
Corms from preflowering and harvested plants can be used for corm multiplication. Jan 04, 2010 banana cultivation in india banana is a globally important fruit crop with 97. Introduction banana is a globally important fruit crop with 97. It is highly desirable to define the procedure needed for a fast rate of propagation in vitro. Banana grows well at temperature range of 15c to 35c with a relative humidity of 75 to. Micropropagation definition of micropropagation by the free.
The shoot tips were cultured on ms media supplemented with different concen trations of bap 0, 2, 4, 6 and 8 mgl with or without iaa at concentration of 0. Thidiazuron in the improvement of banana micropropagation sinwan lee taiwan banana research institute, p. A welldeveloped banana or plantain consists of a clump of meristems of different ages and stages of development. P r e f a c e banana musa sapientum is an important fruit crop in india. They are giant perennial herbs 2 to 9m, some species of which can grow up to 15m tall.
Banana grows well at temperature range of 15c to 35c with a relative humidity of 75 to 85%. This banana file type entry was marked as obsolete and no longer supported file format. Soil nutrient status and fertilizer recommendations soil test assessment of total amounts of nutrients in soil available to crops fertilizer recommendations 3. Micropropagation stages, types, applications, advantages. Micropropagation offers the potential to produce thousands or even millions of plants per annum.
International journal of engineering research and general science volume 2, issue 1, january 2014. Yellow zone managing moko disease of banana in latin. Explants carrying performed vegetative bud suitable for enhanced axillary branching. Micropropagation for production of quality banana planting material in asiapacific vii 5. Banana micropropagation medium with vitamins without nh. The banana file extension is reported to be used for some sort of question export format used by banarama by binary cheese it seems to be obsolete product without support from developer. Asiapacific consortium on agricultural biotechnology. Using shoottip culture introduction bananas including plantains musa spp. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits.
Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Dec 25, 2012 introduction banana is a globally important fruit crop with 97. The effects of auxins and cytokinin on growth and development. The triploid cavendish banana is one of the most important fruit crops for export and local consumption in taiwan. Micropropagation definition of micropropagation by the. Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. Explants are always available, independent of season. International journal of engineering research and general science volume 2, issue 1, january 2014 issn 20912730 61. It is highly desirable to define the procedure needed for a fast rate. There are few people in the world who are not familiar with bananas. Aaa is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation. Berkeley and wound each explant on the midri b using a scalpel held vertically. This postharvest profile of banana has been prepared by shri akshay yakub, senior marketing officer under the supervision of shri c r jena, deputy agricultural marketing adviser and assisted by ms.
In this document, the general term banana is used to encompass cultivated varieties. Conventional micropropagation techniques, however, may. Financing institution are also interested to finance of commercial production of banana tissue culture. This is particularly valuable in the case of difficultto.
The rapid spread of fusarium wilt caused by fusarium oxysporum f. Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. The in vivo clonal propagation is often difficult, expensive and even unsuccessful. Important banana commercial varieties grown in asiapacific 49 ii. Application of tissue culture techniques for genetic up gradation of economically important. Content published by the market news service of cirad. Effective sterilization protocol for micropropagation of. International journal of engineering research and general.
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